Cordycepin production by Cordyceps militaris cultivation on spent brewery grains

This is a first report on C.militaris mycelia and fruiting bodies cultivation on Pendant Light solid-state containing spent brewery grains (SBG).Five different strains of C.militaris were cultivated on substrates containing rye grains and 0 to 60% SBG.

Stromata formation on SBG-containing substrates was noticed with two C.militaris strains.All strains failed to grow on substrates containing SBG amounts higher than 50%.Highest (10.

42 mg/g) cordycepin concentration in cultivating substrate was determined with strain CM2 on 50% SBG.One gram of CM11 strain fungal biomass was able to produce 787.11 mg/g of cordycepin.SBG as a byproduct represent a readily available, low price substrate for cordycepin solid-state Nest of Tables production.

Obtained concentrations of cordycepin are so far the highest reported concentrations obtained on solid-state substrates therefore we can talk about cordycepin hyperproduction.

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